English: Time-Lapse Data for PhOTO-M Membrane Dynamics Visualization During Late Gastrulation. This video shows each frame of the time-lapse summarized in Figure 2 in the text. (A) H2B-Cerulean intensity data (blue), (B) unconverted memb-Dendra2 intensity data (green), (C) segmented photoconverted memb-Dendra2 intensity data (red). Membranes are dynamic throughout the time-lapse, starting as a tight cluster of cells and eventually moving apart laterally and away from the developing head toward the ventral side of the embryo as the time-lapse progresses. (D) Merged image of panels (A–C), which also includes segmented nuclear data (multi-colored surfaces) within each of the photoconverted membranes. Scale bar is 50µm.